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Best final results are received when restricting extended cell exposure to ambient temperature situations. Look at keeping unused cells in a humidified incubator with 5% CO2 at 37°C when doing bigger experiments.
Flow cytometry may be used to measure the effectiveness of differentiation protocols with the STEMdiff™ system, by checking out specific marker expression at Every single stage from the differentiation process. It can be useful for other programs, together with cell sorting, immunophenotyping, and purity assessment.
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This merchandise is designed for use in the next exploration place(s) as Portion of the highlighted workflow phase(s). Explore these workflows To find out more regarding the other items we provide to assistance Just about every study place. Research Location Workflow Levels
The readings can be performed before the culture is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
EasySep™ is a quick and simple immunomagnetic cell separation technologies that permits you to isolate nearly any cell varieties from many different sample sources in as tiny as 8 minutes. This video describes the EasySep™ technology.
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Note: For an extremely confluent lifestyle, cultures could be a little bit 가슴수술 more clumpy in the course of dissociation. To beat this, Carefully scrape the monolayer which has a pipette tip following including the dissociation reagent to aid the dissociation through incubation.
Yes, you’ll discover the move-by-stage protocol for TEER measurement to evaluate the epithelial barrier integrity in ALI cultures below.
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